Rosa26-fs-BirA
This mouse is generated from Rosa26-fs-TRAP (below) by crossing to germline Flpe. The Rosa26-fs-TRAP mouse is available from Jax.
- Zhou, P., Zhang, Y., Ma, Q., Gu, F., Day, D.S., He, A., Zhou, B., Li, J., Stevens, S.M., Romo, D., and Pu, W.T. 2013. Interrogating translational efficiency and lineage-specific transcriptomes using ribosome affinity purification. Proc Natl Acad Sci U S A .
Rosa26-fs-TRAP
L10a-GFP knocked into Rosa26 locus and blocked by a floxed stop cassette. Used for translating ribosome affinity purification of mRNA from Cre-labeled cells.
Available from Jax.
- Zhou, P., Zhang, Y., Ma, Q., Gu, F., Day, D.S., He, A., Zhou, B., Li, J., Stevens, S.M., Romo, D., and Pu, W.T. 2013. Interrogating translational efficiency and lineage-specific transcriptomes using ribosome affinity purification. Proc Natl Acad Sci U S A .
Rosa26-CAG-TRAP
L10a-GFP knocked into Rosa26 locus and constitutively activated (no Cre needed). Used for translating ribosome affinity purification of mRNA from all cells.
Available from Jax.
- Zhou, P., Zhang, Y., Ma, Q., Gu, F., Day, D.S., He, A., Zhou, B., Li, J., Stevens, S.M., Romo, D., and Pu, W.T. 2013. Interrogating translational efficiency and lineage-specific transcriptomes using ribosome affinity purification. Proc Natl Acad Sci U S A .
Wt1-CreERT2
CreERT2 knocked into Wt1 locus. Marks genitourinary system and mesothelium.
Available from Jax.
- Zhou, B., Ma, Q., Rajagopal, S., Wu, S. M., Domian, I., Rivera-Feliciano, J., Jiang, D., von Gise, A., Ikeda, S., Chien, K. R. and Pu, W. T. (2008) Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454, 109-113
Wt1-GFPCre
GFPCre knocked into Wt1 locus. Marks genitourinary system and mesothelium.
Available from Jax.
- Zhou, B., Ma, Q., Rajagopal, S., Wu, S. M., Domian, I., Rivera-Feliciano, J., Jiang, D., von Gise, A., Ikeda, S., Chien, K. R. and Pu, W. T. (2008) Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454, 109-113
GATA4-FLAP
GATA4 cDNA knocking, floxed, followed by promoter less AP. Cre excises GATA4 cDNA and activates AP.
Available from MMRRC.
- Ma*, Q., Zhou*, B. and Pu, W. T. (2008) Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 323, 98-104. *equal contributions PDF
GATA4-S105A
Knockin GATA4 allele with ERK phosphorylation site ablated.
Will be available at Jax
- van Berlo JH, Elrod JW, Aronow BJ, Pu WT, Molkentin JD. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A. 2011;108:12331-12336.
GATA4-flag-bio
Knockin GATA4 allele with C-terminal flag-bio tags, where bio is in vivo biotinylated by BirA.
Will be available at Jax (shipment pending)
- He A, Shen X, Ma Q, Cao J, von G, A., Zhou P, Wang G, Marquez VE, Orkin SH, Pu WT. PRC2 directly methylates GATA4 and represses its transcriptional activity. Genes & Development. 2012;26:37-42.